Reconstruction of Neuronal Morphology
Reconstruction of Neuronal Morphology
This chapter, which is concerned with the techniques currently in use for obtaining morphological parameters of neurons, presents suggestions for decreasing the errors introduced during the process. It evaluates the reconstruction techniques that employ both open-source and commercial software programs for obtaining 3-D reconstructions. The chapter suggests that the direct tracing from whole-mount preparations in which the entire cell is contained in a thick section or a ganglion, or tracing from a series of confocal images of a fluorescently labeled cell are the two basic techniques employed to reconstruct neurons at the light microscopy level. It also shows that Cell Centered Database, NeuroMorpho.Org, and Synapse Web are examples of archives of neuronal morphology.
Keywords: neurons, reconstruction techniques, software programs, ganglion, light microscopy, Cell Centered Database, NeuroMorpho.Org, Synapse Web
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