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Trace Metals and Infectious Diseases$
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Jerome O. Nriagu and Eric P. Skaar

Print publication date: 2015

Print ISBN-13: 9780262029193

Published to MIT Press Scholarship Online: May 2016

DOI: 10.7551/mitpress/9780262029193.001.0001

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Emerging Strategies in Metalloproteomics

Emerging Strategies in Metalloproteomics

Chapter:
(p.311) 18 Emerging Strategies in Metalloproteomics
Source:
Trace Metals and Infectious Diseases
Author(s):

Peter-Leon Hagedoorn

Publisher:
The MIT Press
DOI:10.7551/mitpress/9780262029193.003.0018

The impact of changing metal ion concentrations on infection with a pathogenic microorganism has been established in several cases. To understand the molecular basis of the interplay between metals in the host and the pathogen in infectious diseases, metalloproteomics may prove to be a very powerful approach. Metalloproteomics is the comprehensive analysis of all metal-binding and metal-containing proteins in a biological sample. Strategies in metalloproteomics can be divided into experimental and computer-based approaches. Samples that have been analyzed using metalloproteomics strategies are cell lysates, subcellular fractions, and recombinantly expressed proteins from structural genomics platforms. Samples can be enriched in metal-binding protein by using Immobilized Metal ion Affinity Chromatography (IMAC). Computer-based strategies have been developed to predict metalloproteomes from genomes based on information from literature resources and different genomic and protein structural databases. Many of the methods presented here are comprehensive in nature. The interplay of computer-based and experimental strategies in metalloproteomics will significantly advance the field in the near future.

Keywords:   infectious disease, metalloproteomics, experimental approaches, computer-based approaches, immobilized metal ion affinity chromatography

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