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Trace Metals and Infectious Diseases$
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Jerome O. Nriagu and Eric P. Skaar

Print publication date: 2015

Print ISBN-13: 9780262029193

Published to MIT Press Scholarship Online: May 2016

DOI: 10.7551/mitpress/9780262029193.001.0001

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PRINTED FROM MIT PRESS SCHOLARSHIP ONLINE (www.mitpress.universitypressscholarship.com). (c) Copyright The MIT Press, 2021. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in MITSO for personal use.date: 01 August 2021

Methods and Technologies for Studying Metals in Biological Systems

Methods and Technologies for Studying Metals in Biological Systems

Chapter:
(p.341) 20 Methods and Technologies for Studying Metals in Biological Systems
Source:
Trace Metals and Infectious Diseases
Author(s):

Wolfgang Maret

Joseph A. Caruso

Christopher H. Contag

David P. Giedroc

Peter-Leon Hagedoorn

Andreas Matusch

Eric P. Skaar

Richard B. Thompson

Publisher:
The MIT Press
DOI:10.7551/mitpress/9780262029193.003.0020

An extensive summary is provided on the methods and methodology available to analyze and image total metals in biological systems as well as to assess their speciation, in particular in metalloproteomes and as free metal ion concentrations. Discussion focuses on instrumental methods for analysis and separation and how they are complemented by genetic and bioinformatics approaches. The treatment of methods follows increased complexity and experimental challenges: from lysates and fluids, to cells and tissues, to living cells and animals, and finally to the prospects of applying extent technology to investigations in humans. Presently, method sensitivity is not sufficient for analysis of metals in the pathogen in vivo. Future analytical needs are presented to address metal ions in host–pathogen interactions for diagnosis and treatment of infectious disease. The material is presented in the context of both redistribution of metals in the host and known mechanisms of warfare to acquire the transition metal ions that are essential for growth and survival of either host or pathogen.

Keywords:   element imaging techniques, metalloproteomes, genetic approaches, bioinformatics approaches, measurement methods, host–pathogen interactions

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