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ProtocellsBridging Nonliving and Living Matter$
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Steen Rasmussen, Mark A. Bedau, Liaohai Chen, David Deamer, David C. Krakauer, Norman H. Packard, and Peter F. Stadler

Print publication date: 2008

Print ISBN-13: 9780262182683

Published to MIT Press Scholarship Online: August 2013

DOI: 10.7551/mitpress/9780262182683.001.0001

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Assembly of a Minimal Protocell

Assembly of a Minimal Protocell

(p.124) (p.125) 6 Assembly of a Minimal Protocell

Steen Rasmussen

James Bailey

James Boncella

Liaohai Chen

Gavin Collis

Stirling Colgate

Michael DeClue

Harold Fellermann

Goran Goranović

Yi Jiang

Chad Knutson

Pierre-Alain Monnard

Fouzi Mouffouk

Peter E. Nielsen

Anjana Sen

Andy Shreve

Arvydas Tamulis

Bryan Travis

Pawel Weronski

William H. Woodruff

Jinsuo Zhang

Xin Zhou

Hans Ziock

The MIT Press

This chapter explores the various efforts to synthesize protocell precursors. It introduces the two possibilities for replicating the nucleic acid genome of a synthetic protocell: spontaneous replication and RNA replicase. It also discusses the engineering of a suitable dynamic membrane compartment and the importance of understanding the mechanism of vesicle growth for optimizing protocell growth procedures. Once the replication of the membrane compartment and the nucleic acid component has been engineered, they will be integrated to produce protocell precursors.

Keywords:   protocell precursors, nucleic acid genome, synthetic protocell, spontaneous replication, RNA replicase, vesicle growth, protocell growth

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