- Title Pages
- Preface
- Acknowledgments
- Introduction
-
1 The Early History of Protocells: The Search for the Recipe of Life -
2 Experimental Approaches to Fabricating Artificial Cellular Life -
3 Semisynthetic Minimal Cells: New Advancements and Perspectives -
4 A Roadmap to Protocells -
II INTEGRATION -
5 Steps Toward a Synthetic Protocell -
6 Assembly of a Minimal Protocell -
7 Population Analysis of Liposomes with Protein Synthesis and a Cascading Genetic Network -
8 Constructive Approach to Protocells: Theory and Experiments -
9 Origin of Life and Lattice Artificial Chemistry -
10 Models of Protocell Replication -
11 Compositional Lipid Protocells: Reproduction without Polynucleotides -
12 Evolutionary Microfluidic Complementation Toward Artificial Cells -
III COMPONENTS -
13 Self-Replication and Autocatalysis -
14 Replicator Dynamics in Protocells -
15 Peptide Nucleic Acids as Prebiotic and Abiotic Genetic Material -
16 The Core of a Minimal Gene Set: Insights from Natural Reduced Genomes -
17 Parasitism and Protocells: Tragedy of the Molecular Commons -
18 Forming the Essential Template for Life: The Physics of Lipid Self-Assembly -
19 Numerical Methods for Protocell Simulations -
20 Core Metabolism as a Self-Organized System -
21 Energetics, Energy Flow, and Scaling in Life -
IV BROADER CONTEXT -
22 Gánti’s Chemoton Model and Life Criteria -
23 Viral Individuality and Limitations of the Life Concept -
24 Nonlinear Chemical Dynamics and the Origin of Life: The Inorganic-Physical Chemist Point of View -
25 Early Ancestors of Existing Cells -
26 Prebiotic Chemistry, the Primordial Replicator, and Modern Protocells -
27 Cell-like Entities: Scientific Challenges and Future Applications -
28 Social and Ethical Issues Concerning Protocells - Glossary
- About the Authors
- Index
Assembly of a Minimal Protocell
Assembly of a Minimal Protocell
- Chapter:
- (p.124) (p.125) 6 Assembly of a Minimal Protocell
- Source:
- Protocells
- Author(s):
Steen Rasmussen
James Bailey
James Boncella
Liaohai Chen
Gavin Collis
Stirling Colgate
Michael DeClue
Harold Fellermann
Goran Goranović
Yi Jiang
Chad Knutson
Pierre-Alain Monnard
Fouzi Mouffouk
Peter E. Nielsen
Anjana Sen
Andy Shreve
Arvydas Tamulis
Bryan Travis
Pawel Weronski
William H. Woodruff
Jinsuo Zhang
Xin Zhou
Hans Ziock
- Publisher:
- The MIT Press
This chapter explores the various efforts to synthesize protocell precursors. It introduces the two possibilities for replicating the nucleic acid genome of a synthetic protocell: spontaneous replication and RNA replicase. It also discusses the engineering of a suitable dynamic membrane compartment and the importance of understanding the mechanism of vesicle growth for optimizing protocell growth procedures. Once the replication of the membrane compartment and the nucleic acid component has been engineered, they will be integrated to produce protocell precursors.
Keywords: protocell precursors, nucleic acid genome, synthetic protocell, spontaneous replication, RNA replicase, vesicle growth, protocell growth
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- Title Pages
- Preface
- Acknowledgments
- Introduction
-
1 The Early History of Protocells: The Search for the Recipe of Life -
2 Experimental Approaches to Fabricating Artificial Cellular Life -
3 Semisynthetic Minimal Cells: New Advancements and Perspectives -
4 A Roadmap to Protocells -
II INTEGRATION -
5 Steps Toward a Synthetic Protocell -
6 Assembly of a Minimal Protocell -
7 Population Analysis of Liposomes with Protein Synthesis and a Cascading Genetic Network -
8 Constructive Approach to Protocells: Theory and Experiments -
9 Origin of Life and Lattice Artificial Chemistry -
10 Models of Protocell Replication -
11 Compositional Lipid Protocells: Reproduction without Polynucleotides -
12 Evolutionary Microfluidic Complementation Toward Artificial Cells -
III COMPONENTS -
13 Self-Replication and Autocatalysis -
14 Replicator Dynamics in Protocells -
15 Peptide Nucleic Acids as Prebiotic and Abiotic Genetic Material -
16 The Core of a Minimal Gene Set: Insights from Natural Reduced Genomes -
17 Parasitism and Protocells: Tragedy of the Molecular Commons -
18 Forming the Essential Template for Life: The Physics of Lipid Self-Assembly -
19 Numerical Methods for Protocell Simulations -
20 Core Metabolism as a Self-Organized System -
21 Energetics, Energy Flow, and Scaling in Life -
IV BROADER CONTEXT -
22 Gánti’s Chemoton Model and Life Criteria -
23 Viral Individuality and Limitations of the Life Concept -
24 Nonlinear Chemical Dynamics and the Origin of Life: The Inorganic-Physical Chemist Point of View -
25 Early Ancestors of Existing Cells -
26 Prebiotic Chemistry, the Primordial Replicator, and Modern Protocells -
27 Cell-like Entities: Scientific Challenges and Future Applications -
28 Social and Ethical Issues Concerning Protocells - Glossary
- About the Authors
- Index
