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ProtocellsBridging Nonliving and Living Matter$
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Steen Rasmussen, Mark A. Bedau, Liaohai Chen, David Deamer, David C. Krakauer, Norman H. Packard, and Peter F. Stadler

Print publication date: 2008

Print ISBN-13: 9780262182683

Published to MIT Press Scholarship Online: August 2013

DOI: 10.7551/mitpress/9780262182683.001.0001

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Constructive Approach to Protocells: Theory and Experiments

Constructive Approach to Protocells: Theory and Experiments

(p.169) 8 Constructive Approach to Protocells: Theory and Experiments

Kunihiko Kaneko

The MIT Press

This chapter discusses the quantitative analysis of liposome populations with protein synthesis and cascading genetic network. Protein expression in liposomes involves the synthesis of green fluorescent protein (GFP) by an encapsulated translation system. Flow cytometry is used to estimate the number of GFP molecules synthesized and the internal aqueous volume for each liposome. Results show that protein synthesis in liposomes is estimated to be 520 GFP molecules per gene. An experiment with cascading genetic networks within liposomes show that genetic networks can proceed at rates comparable to protein synthesis by living cells.

Keywords:   liposomes, protein synthesis, cascading genetic network, protein expression, green fluorescent protein, GFP, flow cytometry

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